hNIS-IRES-eGFP Dual Reporter Gene Imaging
نویسندگان
چکیده
منابع مشابه
Dual-modality gene reporter for in vivo imaging.
The ability to track cells and their patterns of gene expression in living organisms can increase our understanding of tissue development and disease. Gene reporters for bioluminescence, fluorescence, radionuclide, and magnetic resonance imaging (MRI) have been described but these suffer variously from limited depth penetration, spatial resolution, and sensitivity. We describe here a gene repor...
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Many in vitro and in vivo applications for transgenesis require co-expression of heterologous genes. The use of internal ribosome entry sites (IRESs) in dicistronic expression vectors enables the expression of two genes controlled by one promoter in target cells or whole organisms. Here we describe the expansion of IRES exploitation to generate multicistronic vectors capable of expressing multi...
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Corresponding Author: E. Mark Haacke, PhD WSU MR Research Facility, HUH-MR Research/Radiology, 3990 John R Street, Detroit, Michigan 48201; E-mail: [email protected]
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To generate a more efficient in vivo reporter and therapeutic gene, we optimized the coding sequence of the human sodium/iodide symporter (NIS) gene by replacing NIS DNA codons from wild type to new codons having the highest usage in human gene translation. The Codon Adaptation Index (CAI), representing the number of codons effective for human expression, was much improved (0.79 for hNIS, 0.97 ...
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Molecular imaging has undergone an explosive advancement in recent years, due to the tremendous research efforts made to understand and visualize biological processes. Molecular imaging by definition assesses cellular and molecular processes in living subjects, with the targets of following metabolic, genomic, and proteomic events. Furthermore, reporter gene imaging plays a central role in this...
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ژورنال
عنوان ژورنال: Molecular Imaging
سال: 2005
ISSN: 1536-0121,1536-0121
DOI: 10.1162/15353500200504193